Study of measuring the apoptosis-necrosis cell cycle by flow cytometry in skin fibroblast cells

Abstract

The aim of the present study is to study the measurement of the apoptosis-necrosis cell cycle by flow cytometry in skin fibroblast cells. In order to investigate the rate of cell death, cells treated with nanodrug and compare it with the cell population of the control (negative) group, the cells were stained with two dyes: V-Annexin and propidium iodide (PI) by the Biolegend kit. During the apoptosis process, phosphatidylserine on the inner surface of the cytoplasmic membrane is transferred to the outer surface of the plasma membrane. In order to determine the effects of nanomedicines in inducing apoptosis or necrosis, the percentage of cells located in each area is calculated by the flowjo flow cytometry software at each repetition. The results clearly show that the percentage of apoptotic cells in cells treated with nanomedicines has increased compared to the control group. Comparative study of cell death (primary-secondary apoptosis-necrosis) in the treatment and control groups. The rate and type of cell death in the treatment and control groups showed that there is no significant difference between the types of cell death. At 24 hours. Comparative study of cell death (primary-secondary apoptosis-necrosis) in the treatment and control groups showed that there is no significant difference between the types of cell death. At 48 hours.